Methods

Introduction

• environmental factors, including the microbiome, are the most important contributors to PTB, particularly among women of African ancestry
• Microbe-induced inflammation resulting from urinary tract infection, sexually transmitted infections, including trichomoniasis, or bacterial vaginosis is thought to be a cause of PTB
• Recent reports of the microbiome in pregnant women have suggested that the composition of the vaginal microbiome has a significant population-specific impact on PTB risk.
• Consequently, population-specific studies may be required to assess the broad impacts of the vaginal microbiome on risk of PTB and to identify contributing taxa that may be carried by only a small subset of women

Results

The Multi-Omic Microbiome Study: Pregnancy Initiative

• The longitudinal iHMP study, the Multi-Omic Microbiome Study

  • 1,572 pregnancies = 992 + 580
    • 992 - from clinics associated with the Research Alliance for Microbiome Science (RAMS) Registry, based at Virginia Commonwealth University (VCU) in Virginia,
    • 580 - pregnancies from sites associated with the Global Alliance to Prevent Prematurity and Stillbirth (GAPPS) in Washington State.
  • The resource features two comprehensive datasets of integrated microbiome and host functional properties measured longitudinally in pregnancy and the perinatal period
    1. the MOMS-PI Preterm Birth (PTB) study dataset generated from a case–control study of 45 women predominantly of African ancestry, who delivered spontaneously preterm, and 90 case-matched women who delivered at term
    2. the MOMS-PI Preterm Birth (PTB) study dataset generated from a case–control study of 45 women predominantly of African ancestry, who delivered spontaneously preterm, and 90 case-matched women who delivered at term

  < About Data From >

  1. 16S ribosomal RNA (rRNA) taxonomic profiles from 6,452 samples from pregnant women and 2,753 samples from neonates (total 9205)
  2. metagenome profiles from 930 samples from pregnant women and 146 samples from neonates
  3. metatranscriptome profiles from 297 samples from pregnant women
  4. cytokine profiles from 1,223 samples from pregnant women and 173 samples from neonates
  5. lipid profiles from 63 samples from pregnant women

Vaginal microbiome profiles show PTB-associated trends

• PTB
  • 45 single gestation pregnancies
    • sponteous PTB (23-36 weeks 6 days of gestational age)
  • 90 single gestation pregnancies
    • extended through term (≥ 39 weeks)
• TB
  • 2TB : 1PTB
    • 최소 18weeks
    • subject당 visit num 평균 = 70
    • The respective mean and median gestational age at delivery was 34, 0/7 and 35, 6/7 for the PTB group and 40, 0/7 and 39, 6/7 for the TB group.

Metagenomic assembly of reference genomes of bacterial taxa associated with PTB.

Bacterial taxa associated with PTB in metagenomic and metatranscriptomic data